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KMID : 0380020060210060439
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.439 ~ p.443
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The Evaluation of Biocompatibility of Collagen/Chondroitin Sulfate Sponge as a Scaffold for Corneal Stromal Layer
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Jang In-Keun
Song Kye-Yong
Kim Jae-Chan
Park Jung-Keug
Ahn Jae-Il
Seo Young-Kwon
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Abstract
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Biocompatibility and tissue regenerating capacity are essential characteristics in the design of collagenous biomaterials for tissue engineering. Attachment of glycosaminoglycans to collagen may add to these characteristics by creating an appropriate micro-environment. In this study, porous type I collagen matrices were crosslinked using dehydrothermal treatment and 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide, in the presence and absence of chondroitin sulfate (CS). The scaffold like discs in 3 mm diameter were inserted into the intralamellar stromal pockets of rabbit cornea. In 8 weeks of follow up, clinical evaluation including corneal neovascularization, opacity and transparency of the graft scaffold was performed, and the inflammatory reaction and migration of corneal fibroblast were evaluated histologically. No inflammation, neovascularization and opacity in any of the implant were observed. CS increased the corneal fibroblast invasion and the transparency. It is concluded that the type I collagen sponge showed a biocompatibility in corneal stromal layer and addition of CS slightly improved the quality of the bioartificial corneal stromal layer. These results could be useful for the development of corneal substitutes.
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KEYWORD
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Collagen sponge, corneal stroma, biocompatibility, chondroitin sulfate
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